|Application:||Gene expression microarray analysis|
|Number of samples:||11|
|Release date:||Feb 18 2010|
|Last update date:||Mar 21 2012|
|Diseases:||Arrhythmias, Cardiac, Atrial Fibrillation, Brain Ischemia, Heart Failure, Death, Sudden, Cardiac|
|Dataset link||Molecular Remodeling of Ion Channels in Human Atrial and Ventricular Myocytes Associated with Ischemic Cardiomyopathy|
In our experiments we used 3 healthy hearts rejected from transplantation procedure and explanted ICM hearts from three male patients. Tissue samples were dissected from left ventricle and left atrial appendages. Atrial and ventricular myocytes were laser-capture microdissected from serial 7-8-µm thick cryostat sections. Individual cellular total RNA samples were analyzed on custom-built Human Ion Channel Splice Arrays slides (ExonHit) manufactured on the Ion Channel Splice Array sv1.1 platform representing 287 human SM, including 248 alternatively spliced ones in total 1655 splicing events and supplemented with capabilities to recognize connexins and ryanodine receptors.