Dataset features

Specifications


Dataset type: Expression profiling by SAGE, transcription profiling by SAGE, Transcriptome or Gene expression
Number of samples: 2
Release date: Mar 22 2011
Last update date: Mar 22 2012
Access: Public
Diseases: Dengue, Immune System Diseases, Salivary Gland Diseases
Dataset link Induction of a peptide with activity against a broad spectrum of pathogens in the Aedes aegypti salivary gland, following infection with dengue virus

Experimental Protocol


Infectious blood meals were offered to 3-day-old, adult, female Ae. aegypti Liverpool mosquitoes using a silicone membrane feeder system (Alto et al., 2005). Human blood was combined with DENV-2 16681 to provide a blood meal titer of 5.106 plaque forming units (PFU)/ml. At different time-points after the blood meal, salivary glands were dissected in acid guanidium thiocyanate-phenol-chloroform (RNAble; Eurobio, France) or phosphate-buffered saline (PBS), and the samples were frozen at -70°C until use.

Repositories


GEO

GSE21528

ArrayExpress

E-GEOD-21528

BioProject

PRJNA125945

Download


Contact


David Piquemal

Dataset Statistics

info

Citations per year

Dataset publication