|Dataset type:||Expression profiling by SAGE, transcription profiling by SAGE, Transcriptome or Gene expression|
|Number of samples:||2|
|Release date:||Mar 22 2011|
|Last update date:||Mar 22 2012|
|Diseases:||Dengue, Immune System Diseases, Salivary Gland Diseases|
|Dataset link||Induction of a peptide with activity against a broad spectrum of pathogens in the Aedes aegypti salivary gland, following infection with dengue virus|
Infectious blood meals were offered to 3-day-old, adult, female Ae. aegypti Liverpool mosquitoes using a silicone membrane feeder system (Alto et al., 2005). Human blood was combined with DENV-2 16681 to provide a blood meal titer of 5.106 plaque forming units (PFU)/ml. At different time-points after the blood meal, salivary glands were dissected in acid guanidium thiocyanate-phenol-chloroform (RNAble; Eurobio, France) or phosphate-buffered saline (PBS), and the samples were frozen at -70°C until use.
Citations per year