Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing, Genome binding/occupancy profiling by high throughput sequencing
Number of samples: 22
Release date: Feb 16 2019
Last update date: Feb 19 2019
Access: Public
Dataset link Allele-specific expression profiling of imprinted genes in mouse isogenic pluripotent tissues and cell lines

Experimental Protocol


We include RNA-Seq on conventional B6D2F1 ESCs derived from fertilized embryos, three ESC lines derived from nuclear transfer embryos generated using B6D2F1 mouse embryonic fibroblast (MEF) donor nuclei (ESC-NT1) or B6D2F1 cumulus donor nuclei (ESC-NT2 and ESC-NT3), as well as three ESC lines derived from PGA embryos generated using B6D2F1 oocytes (ESC-PGA). Also included is RNA-Seq of three fertilized B6D2F1 EpiSC lines, three EpiSC lines derived from nuclear transfer embryos generated using a B6D2F1 cumulus donor nuclei (EpiSC-NT) and two EpiSC-PGA lines. For fertilized ESCs, ESC-NTs and ESC-PGAs we include 1 replica each of H3K4me3 and H3K27me3 ChIP-Seq epigenetic profiling.

Repositories


GEO

GSE101292

ENA

SRP111681

BioProject

PRJNA393966

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Contact


Hendrik Marks