Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 2
Release date: Sep 22 2014
Last update date: Dec 22 2014
Access: Public
Dataset link Sequence specificity incompletely defines the genome-wide occupancy of Myc

Experimental Protocol


Two protein binding microarray (PBM) experiments were performed: one for the heterodimer of the human transcription factors c-Myc and Max, and one for the Max-Max homodimer. Briefly, 4x44K arrays (Agilent Technologies; AmadID 015681) containing the ‘all 10-mer’ universal PBM design were used. Arrays were incubated with a PBS buffer based protein mixture of wither 10nM His-tagged Myc-Max heterodimer or 10nM His-tagged Max-Max homodimer, 2% milk, 200ng/µL BSA, 50ng/µL Salmon Testes DNA, and 0.02% TX-100. Bound protein was tagged with 10ng/µL anti-His antibody conjugated to Alexa 488 (Qiagen; 35310) in PBS with 2% milk. Data were analyzed to obtain fluorescence intensities for all 8mers. The PBM protocol is described in Berger et al., Nature Biotechnology 2006 (PMID 16998473).

Repositories


GEO

GSE58570

ArrayExpress

E-GEOD-58570

BioProject

PRJNA252907

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Contact


Raluca Gordan

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