Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 59
Release date: Oct 7 2008
Last update date: Aug 28 2018
Access: Public
Dataset link Gene expression profiling in the lung and liver of low and high dose Perfluorooctanoic Acid exposed mouse fetuses

Experimental Protocol


Experiment 1: High Dose: Thirty timed-pregnant CD-1 mice were orally dosed from gestation day 1-17 with either 0, 5, or 10 mg/kg/day PFOA in water. At term, fetal lung and liver were collected, total RNA prepared, and samples pooled from three fetuses per litter. Five biological replicates consisting of individual litter samples were then evaluated for treatment group using Affymetrix mouse 430_2 microarrays. Experiment 2: Low Dose: Thirty timed-pregnant CD-1 mice were orally dosed from gestation day 1-17 with either 0, 1, or 3 mg/kg/day PFOA in water. At term, fetal lung and liver were collected, total RNA prepared, and samples pooled from three fetuses per litter. Five biological replicates consisting of individual litter samples were then evaluated for treatment group using Affymetrix mouse 430_2 microarrays (note one microarray from the lung + 1mg/kg/day dose group was excluded from the study due to high background). Please note that each dose experiment had separate concurrent controls.

Repositories


GEO

GSE13044

ArrayExpress

E-GEOD-13044

BioProject

PRJNA109955

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Contact


Mitchell Rosen
Mitchell B. Rosen