Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: May 14 2012
Last update date: May 15 2017
Access: Public
Diseases: Influenza, Human
Dataset link Acute host transcriptonal response to A/Texas/36/91 influenza and A/Texas/36/91 influenza containing the NS1 gene from r1918 in A549 cells

Experimental Protocol


A549 cells were infected at a MOI=2 with either A/Texas/36/91 (Tx/91) or A/Texas/36/91 (Tx/91) containing the NS1 gene from r1918 influenza. Virus was allowed to bind to cells for 1 h at 4°C in serum-free infection medium supplemented with trypsin (1 g/ml). Mock-infected cells were treated with allantoic fluid instead of virus. Three replicate wells were used for each infection condition at each time point. Cells were harvested for array analysis at 2, 6, and 24hr post-infection. Cells from three individual cultures were pooled for each condition for microarray.

Repositories


GEO

GSE37829

ArrayExpress

E-GEOD-37829

BioProject

PRJNA165851

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Contact


Michael Katze
Richard Roy Green