Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Mar 3 2018
Last update date: Mar 4 2018
Access: Public
Diseases: Influenza, Human, Respiratory Tract Infections, Porcine Reproductive and Respiratory Syndrome
Dataset link Comparison of the transcriptome response within the tracheobronchial lymph node following infection with Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus 2 or Swine Influenza A Virus.

Experimental Protocol


Differentially expressed tags (with respect to control) at all time points were ascertained. The experimental results were integrated with previous studies to develop a robust model of swine respiratory virus infection. Here we identified and compared gene expression changes in tracheobronchial lymph nodes (TBLN) following viral infection using Digital Gene Expression Tag Profiling (DGETP). Pigs were infected with 1 x 10^5 cell culture infectious dose 50% per pig of PRRSV (strain SDSU73), porcine circovirus type 2 (PCV2; group 2), or swine influenza A virus (IAV-S; A/SW/OH/511445/2007). Pigs were allotted to one of 4 treatment groups: sham inoculated control, PRRSV-challenge, PCV2-challenge, or IAV-S-challenge.

Repositories


GEO

GSE111378

ENA

SRP133848

BioProject

PRJNA436740

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Contact


Laura Miller