Dataset features


Dataset type: Other
Number of samples: 24
Release date: Oct 27 2010
Last update date: Mar 22 2012
Access: Public
Diseases: Leukemia, Hairy Cell, Neoplasms
Dataset link MicroRNA profiling analysis of differences between bone marrow mouse stem cells versus leukemic and teratocarcinomic cell lines

Experimental Protocol

We isolated the bone marrow cells from mice by femur and tibial flush, then wash and collected. We isolate the same number of cells from fresh cultures of different cell lines types at passage 3, then wash and collected. Total RNA was extracted for the TaqMan Low Density Array v2.0.RNA quality was assessed using Nanodrop for concentration and purity (OD 260/280 and 260/230) and the Agilent Bioanalyzer 2100 for integrity (RNA integrity number (RIN) > 9). Normalization was performed with common endogenous controls for A card and B card (MummU6, snoRNA135, snoRNA202 reference snRNAs for mouse).Comparative real-time PCR was performed in 3 replicates, including no-template controls.