Computational protocol: The HIV-1 Integrase Mutations Y143C/R Are an Alternative Pathway for Resistance to Raltegravir and Impact the Enzyme Functions

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Protocol publication

[…] Amplicons were purified using the QiaQuick PCR purification (Qiagen). IN sequencing reactions were performed using the BigDye Terminator cycle sequencing kit and run on an ABI3730 automated sequencer (Applied Biosystems). Sequence editing and contig assembly were performed using Sequencher v4.1.4 (Gene Codes Corporation) or SeqScape v2.5 (Applied Biosystems) and HXB2 as a reference. […]

Pipeline specifications

Software tools Sequencher, SeqScape
Application Sanger sequencing
Organisms Human immunodeficiency virus 1
Diseases Machado-Joseph Disease