Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 2
Release date: Sep 26 2011
Last update date: Jul 20 2018
Access: Public
Diseases: Sarcoma, Synovial
Dataset link Genomewide occupancy of the synovial sarcoma-associated SYT-SSX2 oncoprotein in C2C12 myoblasts

Experimental Protocol


C2C12 myoblasts were infected with retrovirus carrying SYT-SSX2-HA-FLAG expression vector. Chromatin immunoprecipitation (ChIP) was performed on cell lysates with either control IgG antibody (Abcam) or anti-FLAG antibody (Sigma). Lysates from 2 independent infections were pooled and used to isolate control ChIP DNA (IgG). DNA from 2 independent FLAG ChIP experiments (each using lysates from 2 independent infections) was pooled for SYT-SSX2 ChIP.

Repositories


GEO

GSE26564

ArrayExpress

E-GEOD-26564

ENA

SRP005276

BioProject

PRJNA142109

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Contact


Josiane Eid