Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 210
Release date: Jan 11 2019
Last update date: Feb 18 2019
Access: Public
Dataset link Circulating Tumor Cells Clustering Triggers DNA Methylation Changes to Enable Metastasis Seeding

Experimental Protocol


The ability of circulating tumor cells (CTCs) to form clusters is key to accelerate the metastatic process. Yet, biological features and vulnerabilities of CTC-clusters remain largely unknown. Here, we profile DNA methylation and RNA expression landscape of single CTCs and CTC-clusters, matched within individual breast cancer patients and mouse models. We find that clustering of CTC leads to a remarkable hypomethylation of binding sites for stemness-related transcription factors. Through a screen with 2’486 FDA-approved compounds, we identify drugs that are able to dissociate CTC-clusters into single cells, leading to DNA re-methylation at critical sites and to a reduction in metastasis-seeding ability. Thus, clustering of CTCs favors accessibility to binding sites of stemness-related transcription factors, and cluster-dissociating compounds enable to suppress the spread of cancer.

Repositories


GEO

GSE111065

ENA

SRP133387

BioProject

PRJNA435739

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