Computational protocol: Identification and characterization of secreted and pathogenesis-related proteins in Ustilago maydis

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Protocol publication

[…] Bioinformatic prediction of subcellular protein localization was done as described (Kamper et al. ). The occurrence of secretory targeting signals (signal peptides) was predicted using signalP (v. 3.0) which takes into account the N-terminal region (70 aa) of the protein sequence (Bendtsen et al. ). ProtComp (v. 6.0; http://www.softberry.com) analyzes the entire protein sequence, and the integral prediction score was used to predict subcellular localization. […]

Pipeline specifications

Software tools SignalP, ProtComp
Application Protein sequence analysis
Organisms Ustilago maydis, Saccharomyces cerevisiae
Diseases Infection