Computational protocol: Exon-primed intron-crossing (EPIC) markers for evolutionary studies of Ficus and other taxa in the fig family (Moraceae)1

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Protocol publication

[…] DNA chromatograms were edited using the SEQUENCHER program (Gene Codes Corporation, Ann Arbor, Michigan, USA). DNA sequences were initially aligned using ClustalX version 1.81 () with default settings, and subsequently aligned manually using Se-Al (). We determined number of polymorphic sites, nucleotide diversity (π), and GC content using MEGA 5 software (). […]

Pipeline specifications

Software tools Sequencher, Clustal W, Se-Al, MEGA
Application Nucleotide sequence alignment
Organisms Arabidopsis thaliana