Computational protocol: Genome Wide Expression Profiling of Cancer Cell Lines Cultured in Microgravity Reveals Significant Dysregulation of Cell Cycle and MicroRNA Gene Networks

Similar protocols

Protocol publication

[…] Microarray analysis was performed with RNA samples from DLD-1 and MOLT-4 cell lines grown under microgravity and under static conditions in replicates. Expression data for each sample was obtained on the Affymetrix GeneChip Human Primeview Array. Hybridization was carried out for a duration of 16 hours at 60 rpm at 48°C and scanned on the GeneChip microarray Scanner 3000 7G. Raw data was extracted after scanning of slides and raw data sets were analyzed using GeneSpring GX 12.6 software followed by differential gene expression (DE), fold change & cluster analysis. […]

Pipeline specifications

Software tools PrimeView, GeneSpring GX
Application Miscellaneous
Organisms Homo sapiens
Diseases Neoplasms, Colorectal Neoplasms