Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 100
Release date: Jun 27 2009
Last update date: Mar 21 2012
Access: Public
Chemicals: Cadmium, Ergosterol, Potassium, Sodium
Dataset link Remodeling of global transcription patterns of C. neoformans genes mediated by stress-activated HOG signaling pathways

Experimental Protocol


There is more than 95% genome homology between JEC21 (Cryptococcus neoformans var. neoformans serotype D) and H99 (Cryptococcus neoformans var. grubii serotype A). Therefore, 100 slides of JEC21 70-mer oligo arrays are used in this analysis. 3 biological replicate experiments are performed. Total RNAs are extracted under 3 conditions (1M NaCl, 20ug/ul Fludioxonil, 2.5mM Hydrogen peroxide) at 3 time points (0time, 30min, 60min) with 4 strains from H99 (wild type, hog1Δ , ssk1Δ , skn7Δ). We use a mix of all the total RNAs from this experiment as the control RNA. We use Cy5 as the sample dye and Cy3 as the control dye. Several samples are dye swapped.

Repositories


GEO

GSE16692

ArrayExpress

E-GEOD-16692

BioProject

PRJNA117373

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Contact


Yong-Sun Bahn