Dataset features

Specifications


Application: DNA methylation array analysis
Number of samples: 24
Release date: Aug 30 2006
Last update date: Mar 16 2012
Access: Public
Taxon: Arabidopsis thaliana
Dataset link High Resolution Mapping and Functional Analysis of the Methylome in Arabidopsis (mCIP, MBD)

Experimental Protocol


All plants used in this study are of the Columbia accession. The met1 and drm1 drm2 cmt3 mutants are previously published. The mutant alleles are: met1-3; drm1-2, drm2-2, cmt3-11. To avoid variability caused by inbreeding, all homozygous mutants were from F1 segregating populations. Plants were grown under continuous light. DNA was extracted from 5 weeks old plants using the Plant DNeasy Kit (QIAGEN) according to manufacturer’s instructions. The entire aerial parts of 2–3 plants were pooled for each 3 biological replicate.

Repositories


GEO

GSE5094

BioProject

PRJNA95343

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Contact


Steve Jacobsen