A reference collection of cell line and xenograft models of proneural, classical and mesenchymal GBM [cell line]

Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation and a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM. Gene expression in 12 matched patient tumour / derived cell line / resultant xenograft samples was measured using Illumina HumanHT-12_V4_0_R2_15002873_B direct hybridisation beadchips.

Dataset features

Information

Specifications

Application:	Gene expression microarray analysis
Number of samples:	12
Release date:	Aug 21 2018
Last update date:	Aug 22 2018
Access:	Public
Diseases:	Glioblastoma, Tumor Lysis Syndrome
Dataset link	A reference collection of cell line and xenograft models of proneural, classical and mesenchymal GBM [cell line]

Experimental Protocol

This experiment measures RNA expression in patient-derived GBM cell lines.

Repositories

GEO

GSE118790

BioProject

PRJNA486807

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Contact

Brett Stringer