Computational protocol: Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani

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Protocol publication

[…] Multiple sequence alignment of LdTopIIIβ sequences from various species was carried out using CLUSTAL W (http://expasy.org/tools). Three-dimensional models of LdTopIIIβ based on the crystal structure of E. coli topoisomerase III were generated using Swiss Prot (http://expasy.org/sprot). The generated files were opened in RasMol (http://www.rasmol.org/). The protein sequences were represented in ribbon format and the active site residues were represented in ball and stick format over the ribbon structure. […]

Pipeline specifications

Software tools Clustal W, RasMol
Databases UniProt ExPASy
Application Drug design
Organisms Leishmania donovani, Saccharomyces cerevisiae