Computational protocol: Infiltrating cells from host brain restore the microglial population in grafted cortical tissue

Similar protocols

Protocol publication

[…] To calculate the disappearance rate of endogenous microglia, numbers of GFP+ cells at 6 h, 12 h, 18 h, 24 h after transplantation were compared with the number of GFP+ cells at 1 h after transplantation under the same imaging area. To compare the density of the microglia in grafted tissue at different time point, a random area in grafted tissue was selected to count the microglia number. To quantitate the percentage of proliferating microglia, three slices from each mouse brain were randomly chosen, and all of the microglia and BrdU-positive microglia in grafted tissue and host part of border region (accumulation area contain large number of both activated microglia and BrdU-positive cells near the grafted tissue in host brain) were counted. All areas are measured by using ImageJ software. Statistical analyses of all dates were done by using IBM SPSS Statistics 19 software (One way ANOVA for three or more groups, and unpaired t-test for two groups). All date are presented as mean ± SEM. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Cerebrovascular Trauma