|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jan 30 2014|
|Last update date:||May 11 2017|
|Diseases:||Deficiency Diseases, Leukemia, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma|
|Dataset link||Effect of genetic Zfx deletion on gene expression in Notch induced T-ALL|
Independent primary Notch induced T-ALL cell lines were created by retroviral transduction of Notch-IC into hematopoietic progenitors carrying the tamoxifen inducible Cre-ER transgene and the Zfx conditional (Zfx fl/y) allele (lines 14843, 14844, 14846). T-ALL cells generated from each line were isolated from moribund mice and transplanted into sublethally irradiated secondary recipients. Ten days after transplant when T-ALL cells had appeared in the blood of the secondary recipients, they were treated by gavage with either Vehicle or Tamoxifen (5 mg /day for three days) to induce Zfx deletion in leukemia cells. 48 hours after the final Tamoxifen treatment, the mice were sacrificed and T-ALL cells were recovered by FACS for microarray studies.