Computational protocol: Astrovirus as a possible cause of congenital tremor type AII in piglets?

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Protocol publication

[…] The purified products were sequenced at Macrogen Europe (Amsterdam, the Netherlands) and the obtained chromatograms were edited using SeqMan (Lasergene 9, DNASTAR Inc., Madison, USA). ClustalW as well as the phylogenetic analysis were carried out using Mega 5 []. The phylogenetic trees were constructed using the Neighbour-joining algorithm with P-distances and with a bootstrap value of 1000. Sequence identity plots were performed using the BioEdit software (http://www.mbio.ncsu.edu/bioedit/bioedit.html). All the sequences from this study used in the phylogenetic analysis are deposited in Genbank (Accession numbers KC790414 to KC790418). […]

Pipeline specifications

Software tools Clustal W, MEGA, BioEdit
Application Phylogenetics
Organisms Sus scrofa, Porcine circovirus 2, Mamastrovirus 3
Diseases Virus Diseases, Demyelinating Autoimmune Diseases, CNS