Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 74
Release date: Oct 5 2016
Last update date: Oct 22 2018
Access: Public
Diseases: Otitis Media, Sleep-Wake Transition Disorders, Anemia, Diamond-Blackfan
Computational protocol: limma, matplotlib, SMART, Bimax, biclust, Biopython, MEME Suite, GeneCodis
Dataset link Distinct gene expression programs during erythropoiesis from adult and cord blood progenitor cells compared to hiPSCs

Experimental Protocol


The study comprised 74 samples in: 16 adult sample groups, in triplicate unless otherwise stated (day (d) 0_CD34+; d4_SEM-B_CD36+,CD71+,CD235a- (2 replicates); d4_SEM-F_CD36+,CD71+,CD235a-; d4_SEM-i_CD36+,CD71+,CD235a-; d7_SEM-B_CD36+,CD71+,CD235a-; d7_SEM-F_CD36+,CD71+,CD235a-; d7_SEM-B_CD36+,CD71+,CD235a+; d7_SEM-F_CD36+,CD71+,CD235a+; d7_SEM-F_CD71+ (5 replicates); d7_SEM-i_CD71+ (5 replicates); d10_SEM-F_CD71+,CD235a+; d12_SEM-F_CD71+,CD235a+; d14_SEM-F_CD71+,CD235a+; d14_SEM-B_CD71+,CD235a+; d14_SEM-F_CD235a+; d14_SEM-i_CD235a+); 4 Cord Blood sample groups (d0_CD34+; d7_SEM-F_CD36+,CD71+,CD235a-; d7_SEM-F_CD36+,CD71+,CD235a+ (in duplicate); d14_SEM-F_CD71+,CD235a+ (in duplicate)); and 4 hiPSC sample groups (d0_CD31+; d0_CD34+, d7_SEM-i_CD71+ (4 replicates); d14_SEM-i_CD235a+). The reference samples used were d0_CD34+ AB-EBs, CB-EBs or hiPSC-EBs as appropriate.

Repositories


GEO

GSE66260

BioProject

PRJNA276276

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Contact


Alison Merryweather-Clarke

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