Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Dec 7 2017
Last update date: Oct 18 2018
Access: Public
Diseases: Urinary Bladder Neoplasms, Neoplasms
Chemicals: Tyrosine
Mutations: p|SUB|S|427|F
Dataset link Bladder Cancer Associated Mutations in RXRA activate Peroxisome Proliferator-Activated Receptors to Drive Urothelial Proliferation

Experimental Protocol


Two independent bladder cancer cell lines, JMSU-1 and 575A, were transfected with retrovirus to generate stable cell lines expressing either human wild-type RXRA (RXRAwt) or a mutant form of RXRA, RXRA S427F (RXRAS427F). RNA-seq was performed in biological triplicate on RXRAwt-expressing cells treated with vehicle or the PPARG agonist pioglitazone (pio), and RXRAS427F-expressing cells treated with vehicle. Fragments were sequenced on an Illumina HiSeq-2500 or HiSeq-3000. Transcriptomes in the wild-type and S427F conditions were compared to establish a causal role of the RXRA hot-spot mutation S427F in the hyper-activation of PPAR singling.

Repositories


GEO

GSE107734

ENA

SRP126243

BioProject

PRJNA421310

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Contact


Vivek Arora

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