Computational protocol: Two Paralogous Genes Encoding Auxin Efflux Carrier Differentially Expressed in Bitter Gourd (Momordica charantia)

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Protocol publication

[…] Sequencing was performed using an ABI PRISM 377 DNA Sequencer according to the manufacturer’s instructions. Amino acid sequences were aligned using the AlignX program of Vector NTI Suite 10.0 Software (Thermo Fisher Scientific Inc., Waltham, MA, USA). The cleavage site of a potential signal peptide was analyzed by SignalP 3.0 []. Polypeptide topology prediction has been performed using the HMMTOP-Server []. […]

Pipeline specifications

Software tools Vector NTI Advance, SignalP, HMMTOP
Applications Protein sequence analysis, Membrane protein analysis
Organisms Arabidopsis thaliana, Cucumis sativus, Solanum lycopersicum