Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 66
Release date: Dec 31 2014
Last update date: Mar 20 2017
Access: Public
Diseases: Neoplasms, Neuroblastoma
Dataset link Stimulating BMP4 signaling via HDAC11 inhibition suppresses malignancy in preclinical models of human neuroblastoma

Experimental Protocol


Agilent data: Common Reference Design with 48 chips including 17 different samples with 3 replicates per sample. Illumina data: BE(2)C neuroblastoma cells were transfected with siRNAs against HDAC11 for 48h, and 96h, respectively. As controls served siRNA transfection with scrambled siRNAs. In parallel, cells were treated for 72h with the pan-HDAC inhibitor HC-toxin. As control for HC-Toxin, cells were tretaed with MeOH solvent. All experiments were performed in triplicates. RNA was prepared from cell lysates using RNeasy (Qiagen) according to manufacturer's instruction. standard procedures. RNA quality was checked with Agilent Bioanalyzer.

Repositories


GEO

GSE20399

BioProject

PRJNA125531

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Contact


Gabriela Salinas-Riester