Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 12
Release date: Dec 31 2018
Last update date: Feb 19 2019
Access: Public
Dataset link Bulk RNA-seq of adult homeostatic microglia from 4 brain regions

Experimental Protocol


TMEM119+ microglia (3000 cells each sample) from a given region were FACS sorted from pooled male animal samples (C57BL/6N) into RLT lysis buffer in Eppendorf tubes. Three replicates were done for each region. Libraries were prepared following the Smart-seq protocol (v4 ultra low input RNA kit). All samples were barcoded and pooled together for Illumina Nextseq sequencing.

Repositories


GEO

GSE123021

ENA

SRP170960

BioProject

PRJNA507265

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Contact


Qingyun Li