|Dataset type:||Expression profiling by high throughput sequencing|
|Number of samples:||12|
|Release date:||Dec 31 2018|
|Last update date:||Feb 19 2019|
|Dataset link||Bulk RNA-seq of adult homeostatic microglia from 4 brain regions|
TMEM119+ microglia (3000 cells each sample) from a given region were FACS sorted from pooled male animal samples (C57BL/6N) into RLT lysis buffer in Eppendorf tubes. Three replicates were done for each region. Libraries were prepared following the Smart-seq protocol (v4 ultra low input RNA kit). All samples were barcoded and pooled together for Illumina Nextseq sequencing.