Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 6
Release date: Sep 4 2018
Last update date: Sep 4 2018
Access: Public
Diseases: Anemia, Neonatal, Lung Diseases
Dataset link Global Long Noncoding RNA and mRNA Expression Changes between Prenatal and Neonatal Lung Tissue in Pigs

Experimental Protocol


Six female Tibetan-Meishan (Patriarchal: Tibetan pig; Matriarchal: Meishan pig) hybrid piglets were used to simulate the adaptation of newborns. The pregnancy period was considered to be 113 days. The fetal group consisted of three piglets obtained from three pregnant sows of similar weights on the last day before birth. The neonatal group consisted of three piglets of similar weight obtained two days after birth from another three pregnant sows. All parents were of a similar-weight and were raised under the same feeding and rearing conditions until caesarean and parturition. Piglet lung tissues were rapidly sampled from each carcass and selectively separated into two parts. One part was fixed with 10% formalin for morphometric analysis, and the other part frozen immediately in liquid nitrogen and stored at −80 °C until required for RNA extraction, sequencing and transcriptome analysis.

Repositories


GEO

GSE117882

ENA

SRP155770

BioProject

PRJNA483488

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Contact


Mingzhou Li