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Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Jun 2 2011
Last update date: Apr 5 2017
Access: Public
Diseases: Neoplasms
Dataset link Expression data from human amnion mesenchymal cells treated with interleukin-1-beta for 1hr and 8hr

Experimental Protocol


Human amnion mesenchymal cells were challenged with 10 ng/ml of interleukin-1-beta for 1 hour (1h) or 8 hours (8h) in serum-free media, while control cells were treated with serum free medium only (veh). The cells (veh, 1h and 8h) were subjected to RNA extraction, quantification, and evaluation, followed by hybridization on Affymetrix GeneChip® Human Genome U133A 2.0 Arrays (Platform GPL571). Experiments were repeated three times for a total of nine samples used for microarray analysis. Three biological replicates were used to ensure that any results were not biased by day-to-day variation in RNA extraction.

Repositories


GEO

GSE26315

ArrayExpress

E-GEOD-26315

BioProject

PRJNA136761

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