Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Jul 1 2011
Last update date: Mar 23 2012
Access: Public
Chemicals: Glucose, Iron
Dataset link Expression profiling of a spf deletion mutant suggests biological roles and mRNA targets for Spot 42 in the fish pathogen Aliivibrio salmonicida

Experimental Protocol


Two-condition experiment, wild type cells (control samples) vs. spf mutants grown in A. salmonicida-specific minimal media (ASMM) (stimulated samples). Samples were prepared by culturing the A. salmonicida wild type and a spf deletion mutant strains at 12 °C in ASMM without any added carbon source to OD 600nm 0.4. At this density glucose to 44.4 mM was added. Samples were collected 15 min after stimulation. Biological replicates for each sample: 3 wild type, 3 spf mutant, independently grown and harvested. One replicate per array.

Repositories


GEO

GSE28087

ArrayExpress

E-GEOD-28087

BioProject

PRJNA139739

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Contact


Erik Hjerde

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