|Application:||Gene expression microarray analysis|
|Number of samples:||16|
|Release date:||Apr 1 2016|
|Last update date:||Aug 13 2018|
|Diseases:||Diabetes Mellitus, Type 2|
|Dataset link||Generation of Stem Cell-Derived β Cells from Type 1 Diabetic Patients|
Differentiated cells were sorted and processed for RNA isolation using the MARIS protocol published previously (PMID: 24516164.) Human induced pluripotent stem cell (hiPSC) line were differentiated into SC-beta cells or dysfunctional, polyhormonal cells (PH). Four biological replicates were assessed with differentiation to both SC-beta and PH cells. Those data were normalized together with and compared to existing, previously published data from Hrvatin et al. (PMID: 24516164) and Pagliuca et al. (PMID: 25303535) from human islet-derived insulin+ cells, undifferentiated HUES8 hES cells, SC-beta cells derived from HUES8 and PH cells derived from HUES8 according to previously published protocols.
Citations per year