Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Apr 1 2016
Last update date: Aug 13 2018
Access: Public
Diseases: Diabetes Mellitus, Type 2
Chemicals: Alloxan, Glucose
Dataset link Generation of Stem Cell-Derived β Cells from Type 1 Diabetic Patients

Experimental Protocol


Differentiated cells were sorted and processed for RNA isolation using the MARIS protocol published previously (PMID: 24516164.) Human induced pluripotent stem cell (hiPSC) line were differentiated into SC-beta cells or dysfunctional, polyhormonal cells (PH). Four biological replicates were assessed with differentiation to both SC-beta and PH cells. Those data were normalized together with and compared to existing, previously published data from Hrvatin et al. (PMID: 24516164) and Pagliuca et al. (PMID: 25303535) from human islet-derived insulin+ cells, undifferentiated HUES8 hES cells, SC-beta cells derived from HUES8 and PH cells derived from HUES8 according to previously published protocols.

Repositories


GEO

GSE70901

ArrayExpress

E-GEOD-70901

BioProject

PRJNA289894

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