Computational protocol: Subthalamic Nucleus Deep Brain Stimulation Does Not Modify the Functional Deficits or Axonopathy Induced by Nigrostriatal α-Synuclein Overexpression

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Protocol publication

[…] Statistical analyses were performed using IBM SPSS Statistics (IBM, Armonk, NY) or GraphPad Prism (La Jolla, CA). Statistical significance for all cases was set at p < 0.05. Levene’s test was used to verify the equal variances assumption, and a correction was used as necessary. To analyze the impact of α-syn overexpression on forelimb akinesia over time, a one-way RM-ANOVA (Fig. ) followed by least significant difference (LSD) post hoc analysis was used. To compare the impact of STN DBS (Active vs. Inactive) on α-syn overexpression-mediated forelimb deficits (Fig. ), a split-plot RM-ANOVA followed by least significant difference post hoc analysis was used. To compare the effects of unilateral α-syn overexpression between hemispheres on various outcome measures within the same rat (Table , Figs , , , ) a paired samples t-test was conducted. A paired samples t-test was also used to analyze the impact of stimulation OFF vs ON (Fig. ) in the same rat. Within subject comparisons are indicated by a connecting line. To compare the effects of No Electrode vs. Active vs. Inactive stimulation between rats on α-syn immunoreactivity (Fig. ) and the effect of Active vs. Inactive stimulation on SNpc neurons (Fig. ) and TH immunoreactivity in the striatum (Fig. ), a one-way ANOVA with least significant difference post hoc analyses was used. To compare the effect of Active vs. Inactive stimulation on total and phosphorylated rpS6 (Fig. ), differences in immunofluorescence that are not visually verifiable, a more stringent Bonferroni post hoc analysis was used following one-way ANOVA. Data collected by qPCR were compared between hemispheres using the Relative Expression Software Tool 384 (REST-384 version 2) calculation software for the relative expression in real-time PCR using Pair-wise fixed reallocation randomization test (Fig. ). Statistical outliers were assessed using the Absolute Deviation from the Median (ADAM) method using the ‘very conservative’ criterion. […]

Pipeline specifications

Software tools SPSS, REST
Applications Miscellaneous, qPCR
Diseases Parkinson Disease, Drug-Related Side Effects and Adverse Reactions
Chemicals Dopamine, 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Oxidopamine