Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 4
Release date: Dec 1 2008
Last update date: Aug 10 2018
Access: Public
Diseases: Neoplasms
Dataset link Downstreams of hnRNP A2 in Colo16 cells

Experimental Protocol


Western blotting was performed to confirm the efficient suppression of hnRNP A2/B1 by shRNA, which was endogenously generated from a pSUPER vector system. A vector carrying a target sequence of firefly was used as a control. The specificity of the hnRNP A2/B1 targeting vector has been established in our previous study [Yaowu He, Melissa A. Brown, Joseph A. Rothnagel, Nicholas A. Saunders, and Ross Smith. Roles of heterogeneous nuclear ribonucleoprotein A/B in cell proliferation. Journal of Cell Science. 2005.118(14):3173-83] For microarray hybridization, the GeneChip Human Genome U133A platform (Affymetrix) used in this study was printed with 22,283 oligonucleotide probe sets representing 18,400 transcripts and variants, including 14,500 known genes. For a probe set, there were typically 8 probe pairs, each containing a perfect match and a mismatch oligonucleotide. Two chips were prepared for each treatment, allowing 4 comparisons between the hnRNP A2-suppressed and the control cells (samples were not paired).

Repositories


GEO

GSE11865

ArrayExpress

E-GEOD-11865

BioProject

PRJNA105671

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Contact


Yaowu He