|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jan 29 2011|
|Last update date:||Mar 22 2012|
|Chemicals:||Amino Acids, Dimethyl Sulfoxide, Nitrates, Oxygen|
|Dataset link||Shewanella oneidensis MR-1 etrA deletion mutant strain expression profiling determines fine-tuning regulatory role in anaerobic metabolism|
Total RNA was extracted from three biological replicate cultures of both S. oneidensis etrA deletion mutant and the wild type strain, grown individually on anaerobic minimal medium supplemented with 2 mM nitrate as the electron acceptor and lactate as the electron donor. Total RNA was collected from mid-log phase cultures. cDNA from each sample was prepared and labeled with Cy-dyes. Three biological replicates per treatment were used for the hybridization of six microarray slides including technical replicates (dye-swap) per experiment.