Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jan 29 2011
Last update date: Mar 22 2012
Access: Public
Chemicals: Amino Acids, Dimethyl Sulfoxide, Nitrates, Oxygen
Dataset link Shewanella oneidensis MR-1 etrA deletion mutant strain expression profiling determines fine-tuning regulatory role in anaerobic metabolism

Experimental Protocol


Total RNA was extracted from three biological replicate cultures of both S. oneidensis etrA deletion mutant and the wild type strain, grown individually on anaerobic minimal medium supplemented with 2 mM nitrate as the electron acceptor and lactate as the electron donor. Total RNA was collected from mid-log phase cultures. cDNA from each sample was prepared and labeled with Cy-dyes. Three biological replicates per treatment were used for the hybridization of six microarray slides including technical replicates (dye-swap) per experiment.

Repositories


GEO

GSE26935

BioProject

PRJNA136091

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Contact


Claribel Cruz-Garcia