Computational protocol: Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation

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Protocol publication

[…] Heatmap construction of differentially expressed proteins in the HFD and LPS groups compared with their respective control was generated using Cluster 3.0 (http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm) and Java Treeview (http://jtreeview.sourceforge.net). MetaCore (GeneGo, St Joseph, MI, USA) and BiNGO were applied to differentially expressed proteins analysed with at least two unique peptides to identify over-represented processes in the modulated proteome of each group compared with their respective control. FDR-corrected P-values <0.05 were considered significant. […]

Pipeline specifications

Software tools TreeView, JTreeView, MetaCore, BiNGO
Application Phylogenetics
Organisms Mus musculus
Chemicals Glycine, Serotonin