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Pipeline publication

[…] rtype often present the same epitope []. This could have considerable implications for T-cell epitopebased vaccination strategies. Previously, Yoshimatsu and Arikawa [] reported that the epitopes of nucleocapsid protein of hantaviruses were immunodominant. They demonstrated this feature using observation on dynamic differences in the properties with monoclonal and polyclonal antibodies binding to epitopes in Yeast two-hybrid assay and competitive ELISA., We analyzed HFRS causing hantavirus genomes to design a candidate T-cell epitope vaccine using immunoinformatics strategies. The peptide epitopes that bind to Class I HLA supertypes and stimulate T-cell immune responses are predicted by the NetMHCpan software. We aimed to identify candidate Tcell epitope(s) conserved in all 5 hantavirus genotypes (Hantaan, Dobrava-Belgrade, Seoul, Gou and Amur) causing HFRS. The epitopes that bind to Class I HLA supertypes that is also cleaved only at the flanking regions by human proteasomes, binding to TAP efficiently was identified by the programs MAPPP and TAPpred respectively. This study enabled the prediction of MHC Class I binding T- cell Epitopes from hantaviruses towards development of a designer peptide vaccine., All available complete S and M segment amino acid (aa) sequences of genotypes Hantaan virus (n=152), Dobrava- Belgrade virus (n=44), Seoul virus (n=102), Gou virus (n=36) and Amur virus (n=9) that causes hemorragic fever with renal syndrome (HFRS) were retrieved from GenBank database [] as of October 2016. The S segment consensus amino acid sequence was 429 and M segment consensus amino acid sequence was 1135 in length. The study design flow chart is shown in ., A consensus aa sequence for each genotype was identified using CLC […]

Pipeline specifications

Software tools NetMHCpan, MAPPP, TAPPred