Computational protocol: First Diagnosed Case of Camelpox Virus in Israel

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Protocol publication

[…] Primers were designed and tested in silico using the Geneious software (Biomatters, version 8.0, Auckland, New Zealand). A multiple alignment analysis of partial genomic regions was performed using the MUSCLE algorithm []. Alignments of complete Poxvirus genomes were performed using the BLASTZ algorithm []. The alignment file was then used to construct a phylogenetic tree for the tested region, using the Mega6 software [], according to the maximum likelihood method. […]

Pipeline specifications

Software tools Geneious, MUSCLE, LASTZ, MEGA
Applications Phylogenetics, Nucleotide sequence alignment
Organisms Camelpox virus, Camelus dromedarius, Human poliovirus 1 Mahoney
Diseases Skin Diseases