Dataset features

Specifications


Application: ChIP-seq analysis, RNA-seq analysis
Number of samples: 42
Release date: Nov 24 2012
Last update date: Oct 10 2018
Access: Public
Diseases: Deficiency Diseases
Dataset link STATs Shape the Active Enhancer Landscape of T Cell Populations

Experimental Protocol


Active enhancer elements were defined as p300-high/H3K4me1-high. Using ChIP-seq, we mapped active enhancer landscapes of two CD4+ T helper cells, Th1 and Th2. To investigate the effect of STAT proteins on this landscape, we profiled active enhancers in the absence of STATs. Overall, STATs deficiency had a major impact on recruitment of p300. In addition, ectopic expression of master regulators T-bet and GATA3 in STAT-deficient cells failed to recover most active enhancers.

Repositories


GEO

GSE40463

ArrayExpress

E-GEOD-40463

ENA

SRP015458

BioProject

PRJNA174565

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