Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 7
Release date: Jun 13 2016
Last update date: Oct 24 2018
Access: Public
Diseases: Deficiency Diseases, Neoplasms
Genes: DICER1, ITGAX, MRC1, IFNG, STAT1, TRIM63, PDCD1
Dataset link DICER controls macrophage polarization and tumor response to immunotherapy

Experimental Protocol


To explore the role of DICER in the development, activation and immunological functions of TAMs, we crossed homozygous LysM-Cre (Clausen et al., 1999) with Dicerlox/lox (Harfe et al., 2005) mice to obtain mice with myeloid-cell-specific Dicer1 gene deletion (LysM-Cre;Dicer–/–, referred to as D–/–). These mice were then backcrossed to LysM-Cre to obtain the control LysM-Cre; Dicer+/+ mice (referred to as D+/+). Both LysM-Cre and Dicerlox/lox mutations were always homozygous in our experiment. We then inoculated Lewis lung carcinoma (LLC) cells subcutaneously (s.c.) in D–/– and control D+/+ mice. Once the tumors were established, we isolated by fluorescence-activated cell sorting (FACS) tumor-associated macrophages (F4/80+ cells).

Repositories


GEO

GSE76356

ArrayExpress

E-GEOD-76356

ENA

SRP067829

BioProject

PRJNA307086

Download


Contact


Chia-Huey Ooi