|Application:||Gene expression microarray analysis|
|Number of samples:||32|
|Release date:||Mar 17 2015|
|Last update date:||Mar 17 2015|
|Chemicals:||Eicosapentaenoic Acid, Arachidonic Acid|
|Dataset link||Vitamin A and arachidonic acid altered the skeletal mineralization in Atlantic cod larvae (Gadus morhua) without any interactions on the transcriptional level|
Four different experimental groups in a two way factorial design were analyzed with different combination of arachidonic acid enrichement (ARA) and vitamin A (VA) supplement. Two oil mixtures were made, named Control and High ARA. Control contained 90% EPAX4510 and 10% EPAX5010, while High ARA contained 65% Cargill ARA oil, 5% EPAX4510 and 30%EPAX5010. The mixtures were then divided in two fractions. VA in the form of retinylpalmitate (Sigma-Aldrich, Oslo, Norway) was added to one fraction from each oil mixture (5 g retinylpalmitate L-1 oil). This gave four different mixtures, namely Control, Control+VA, High ARA and High ARA+VA. These mixtrures were used to enriched rotifers which in turn were subsequently fed to Atlantic cod larvae from 4 days post hatch (dph) to 29 dph. Four tanks per experimental group was used in the factorial design. Atlantic cod larvae were sampled at 17 dph and 29 dph for microarray analysis. RNA from pooled whole animals were reverse transcribed and Cy3-labled and hybridized.