Computational protocol: HOXA5 determines cell fate transition and impedes tumor initiation and progression in breast cancer through regulation of E-cadherin and CD24

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Protocol publication

[…] One million cells were stained with 5 µl of CD24-FITC (BD Pharmigen; clone ML5), 5 µl CD44-PE (BD Pharmigen; clone 515) and 5 µl of 7AAD (BD Pharmingen) to exclude dead cells. Samples were run on the BD FACSCalibur system (Becton Dickinson), and data analyzed using FlowJo software. Cells were sorted on a BD FACSAria SORP and analyzed on a BDLSRII with BD FACSDiva Software (BD Biosciences). […]

Pipeline specifications

Software tools FlowJo, BD FACSDiva
Application Flow cytometry
Organisms Homo sapiens, Homo sapiens/Mus musculus xenograft
Diseases Breast Neoplasms, Carcinoma, Neoplasms
Chemicals Doxycycline, Tretinoin