Computational protocol: Whole genome sequencing of Mycobacterium tuberculosis SB24 isolated from Sabah, Malaysia

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[…] In Malaysia, tuberculosis (TB) remains a major health threat with over 24,000 cases reported annually since 2013 . Among all the states in Malaysia, Sabah has always recorded the highest number of TB cases. Nevertheless, the characteristic of Mycobacterium tuberculosis (M. tuberculosis) strains that are prevailing in Malaysia, mainly in Sabah is poorly understood. Only a limited number of M. tuberculosis genomes in Malaysia have been sequenced . Here, we report the first draft genome sequence of M. tuberculosis, strain SB24 isolated from cerebrospinal fluid (CSF) of a patient diagnosed with tuberculous meningitis (TBM) at a local hospital in Kota Kinabalu, Sabah. The sample was cultured in Middlebrook 7H9 broth supplemented with OADC enrichment (BD) and PANTA antibiotic mixture (BD). Based on in-vitro drug susceptibility testing (DST) using the microscopic observation drug susceptibility (MODS) assay, this isolate was susceptible to both rifampicin (RIF) and isoniazid (INH).The genomic DNA of M. tuberculosis SB24 was extracted according to the method previously described . Whole genome sequencing (WGS) was performed using the Pacific Biosciences RS II Single-Molecule Real Time (SMRT) sequencing technology. The library was prepared according to the 20-kb Template Preparation Using BluePippin™ Size-Selection System. Library was then sequenced on the PacBio RS II sequencing platform (Pacific Biosciences) using DNA/Polymerase Binding Kit P6 v2 (Pacific Biosciences) and DNA Sequencing Reagent Kit 4.0 (Pacific Biosciences). Titration density was 0.08 nM. Template was loaded into SMRT® cell v3 using a Mag Bead Kit. Sequencing was performed using one SMRT cell and a movie of 360 min was taken.The sequence data obtained from the M. tuberculosis SB24 genome was assembled by using the Hierarchical Genome Assembly Process 3.0 (HGAP 3.0) in SMRT Portal v2.3.0 and the assembly was improved using Quiver iteratively in the SMRT portal. After quality-filtering and trimming, 87,044 reads were obtained with a mean length of 3543 bp totaling 308,405,967 bp. The complete genome sequence of M. tuberculosis SB24 was determined with a length of 4,452,489 bp and a G + C content of 65.6%. M. tuberculosis SB24 genome size is 40,957 bp and 48,653 bp longer than the reference strains H37Rv (4,411,532 bp) and M. tuberculosis CDC1551 (4,403,836) respectively.The draft genome of M. tuberculosis SB24 was annotated by using the Rapid Annotation of microbial genomes using Subsystem Technology (RAST) and GeneMarks , . The annotation for the M. tuberculosis SB24 genome sequence using RAST showed 398 subsystems, 4586 coding sequences and 48 RNA genes (). GeneMarks revealed that the genome of M. tuberculosis SB24 found to encode 4402 proteins.The genome of M. tuberculosis SB24 encodes a gene for the Arylsulfatase enzyme (EC 3.1.6.1) which is involved in the metabolism of Sphingolipids (KEGG Pathway 00600), which leads to the production of Sulfur, one of the essential nutrients required in the survival of M. tuberculosis. Under sulfate-limiting environments, bacteria usually synthesize proteins, known as sulfate starvation-induced (SSI) proteins to complement their sulfur requirements . Arylsulfatase enzyme is one of the SSI proteins produced and functionally help in the assimilation of sulfur from environmental sources. M. tuberculosis SB24 genome also encodes several polyketide synthase (pks) genes which are the pks1, pks7 and pks10. These genes involved in the synthesis of dimycocerosyl phthiocerol (DIM) which is necessary for the virulence of M. tuberculosis , .Screening of antibiotic resistance genes using the curated database, the Antibiotic Resistance Genes Database (ARDB) (http://ardb.cbcb.umd.edu/) , M. tuberculosis SB24 was found to be resistant only to aminoglycosides, thus confirming the in-vitro susceptibility of this isolate to RIF and INH.The closest strains of M. tuberculosis SB24 based on comparison of genome sequences using RAST are M. tuberculosis NCGM2209 (score 472), M. tuberculosis UM 1072388579 (score 417) and M. tuberculosis NA-A0008 (score 413). Based on the nucleotide sequence over the alignable portions using the fast sequence alignment tools BLAST , the genomes of M. tuberculosis SB24 was similar to M. tuberculosis HKBS1, M. tuberculosis CCDC5180, M. tuberculosis CCDC5079, M. tuberculosis 18b and M. tuberculosis Beijing-like with 99% similarity. Phylogenetic tree based on the aligned rpoB gene using BLAST showed that M. tuberculosis SB24 strain belongs to the Beijing family.The availability of this genome sequence would enables the study of comparative analysis and phylogenetic study of this isolate with other M. tuberculosis strains and thus give us important insights into the biology and molecular epidemiology of M. tuberculosis strains that are prevalent in Sabah. […]

Pipeline specifications

Software tools RAST, GeneMarkS
Applications Genome annotation, Phylogenetics, WGS analysis
Organisms Homo sapiens
Diseases Tuberculosis, Tuberculosis, Meningeal