Dataset features


Application: Gene expression microarray analysis
Number of samples: 20
Release date: Jul 7 2009
Last update date: Dec 6 2012
Access: Public
Diseases: Muscular Diseases
Dataset link Expression profiling of skeletal muscle in beef cattle treated with steroidal growth promoters

Experimental Protocol

In this study, we analyzed the gene expression profiles of 20 anterior limb muscle samples, 9 collected from untreated controls, 5 collected from cattle treated with Dex and 6 from cattle treated with Dex+Estr using Agilent-015354 Bovine oligo microarray platform (20 arrays, no replicate) based on single-colour detection (Cyanine-3 only). Microarrays are scanned with Agilent scanner G2565BA (barcode on the left, DNA on the back surface, scanned through the glass) at a resolution of 5 microns; all slides are scanned twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%); the scanner software creates a unique ID for each pair of XDR scans and saves it to both scan image files. Feature Extraction 9.5 uses XDR ID to link the pairs of scans together automatically when extracting data. The signal left after all the FE processing steps have been completed is ProcessedSignal that contains the Multiplicatively Detrended, Background-Subtracted Signal










Serena Ferraresso