Dataset features
Specifications
Application: | RNA-seq analysis, sRNA-seq analysis, ChIP-seq analysis |
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Number of samples: | 35 |
Release date: | Oct 2 2012 |
Last update date: | Oct 18 2018 |
Access: | Public |
Diseases: | Neoplasms, Prostatic Neoplasms |
Chemicals: | Androgens |
Computational protocol: | TopHat, HTSeq, edgeR, GOstats, MetaCore, GeneSigDB |
Dataset link | Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions |
Experimental Protocol
LNCaP, C4-2B, or 22RV1 cells were cultured in hormone-free media for 3 days and then treated with ethanol vehicle or DHT (10nM) for 4h or 16h prior to ChIP-seq or RNA-seq assays. For siRNA transfection, cells were transfected with AR siRNA or control siRNA for 3 days prior to RNA-seq assays.
Repositories
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Contact
Keith Decker
Keith F Decker
Dataset Statistics
info
Citations per year
Dataset publication
2012
Nucleic Acids Res
PMCID: 3510497
PMID: 23019221
DOI: 10.1093/nar/gks888
call_split See protocol