Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Mar 1 2018
Last update date: Mar 3 2018
Access: Public
Diseases: Metabolic Diseases
Chemicals: Amino Acids, Fatty Acids, Unsaturated, Pyruvic Acid, Glucuronic Acid
Dataset link The embryo affects day 14 uterine transcriptome depending on the nutritional status in sheep

Experimental Protocol


46 adult multiparous Rasa Aragonesa ewes were housed in individual pens and offered a diet (once daily) that provided 1 x live weight maintenance requirements for 1 month prior to the beginning of the experimental procedures [12]. The estrus cycles were synchronized using intravaginal sponges, which were inserted for 14 days. At the time of sponge insertion, ewes were allocated to one of two planes of nutrition: a control (n= 21; 7.8 MJ of metabolisable energy per ewe), which continued to receive the same diet for 28 days, and a low plane of nutrition (n = 25; 3.9 MJ of metabolisable energy per ewe), which was offered at 50% of estimated daily requirements (pellet and straw) until the end of the experiment. At the time of sponge withdrawal, ewes were injected with 300 IU, i.v., equine chorionic gonadotropin (Intervet, Salamanca, Spain), and the occurrence of estrus (Day 0) was monitored every 8 h. Thirteen control ewes and 18 ewes in the low plane of nutrition were mated to vasectomized or intact rams to establish a cyclic and pregnant group, respectively, within each plane of nutrition

Repositories


GEO

GSE108176

BioProject

PRJNA422883

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Contact


Mario Vailati Riboni