Computational protocol: Coupling between Myogenesis and Angiogenesis during Skeletal Muscle Regeneration Is Stimulated by Restorative Macrophages

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Protocol publication

[…] Total RNAs were extracted from sorted ECs or MPCs using an RNeasy Mini Kit (QIAGEN). RNA integrity was checked on an Agilent Bioanalyzer 2100. Global expression data were obtained using Affymetrix GeneChip Mouse Gene 2.0 ST arrays. The microarray data are publicly available (GEO: GSE103684). Data were robust multichip average normalized and were first controlled and analyzed in an unsupervised way by principal component analysis, and a one-way ANOVA was applied to extract DEGs using PartekGS software. Cluster analysis and enrichment were made using DAVID and Pathway Studio software (the strategy is presented in J). A list of genes expressed by both ECs and MPCs was finalized (). […]

Pipeline specifications

Software tools DAVID, Pathway Studio
Application Protein interaction analysis