Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Jun 20 2012
Last update date: Oct 29 2018
Access: Public
Diseases: Inflammatory Bowel Diseases, Neoplasms, Second Primary
Chemicals: Ionomycin
Dataset link Modulation of gene expression in cultured human intestinal colon explants by probiotic bacteria

Experimental Protocol


Macroscopically healthy colonic intestinal tissue was obtained at surgery from 3 patients. Intestinal explants were treated with PMA and ionomycin for 3 h to induce pro-inflammatory conditions. Then, culture medium was changed and replaced with either medium or medium containing either Lactobacillus casei BL23, Lactobacillus plantarum 299v, or a nonadherent mutant of L. plantarum 299v (A-) and incubated for further 4 hours. In parallel, control intestinal explants were cultured without any treatment of PMA/ionomycin or probiotic bacteria and compared to directly frozen tissue in order to evaluate changes in gene expression which are due solely to the culture conditions.

Repositories


GEO

GSE23630

ArrayExpress

E-GEOD-23630

BioProject

PRJNA129929

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Contact


Gaspar Perez Martinez