|Application:||Gene expression microarray analysis|
|Number of samples:||18|
|Release date:||Jun 20 2012|
|Last update date:||Oct 29 2018|
|Diseases:||Inflammatory Bowel Diseases, Neoplasms, Second Primary|
|Dataset link||Modulation of gene expression in cultured human intestinal colon explants by probiotic bacteria|
Macroscopically healthy colonic intestinal tissue was obtained at surgery from 3 patients. Intestinal explants were treated with PMA and ionomycin for 3 h to induce pro-inflammatory conditions. Then, culture medium was changed and replaced with either medium or medium containing either Lactobacillus casei BL23, Lactobacillus plantarum 299v, or a nonadherent mutant of L. plantarum 299v (A-) and incubated for further 4 hours. In parallel, control intestinal explants were cultured without any treatment of PMA/ionomycin or probiotic bacteria and compared to directly frozen tissue in order to evaluate changes in gene expression which are due solely to the culture conditions.
Gaspar Perez Martinez