|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Oct 29 2008|
|Last update date:||Mar 20 2012|
|Dataset link||Streptococcus agalactiae genes controlled by the MtaR transcription factor|
Three cultures of an mtaR insertional mutant (DS101) and three cultures of the isogenic wild-type strain (COH1) were grown in a chemically-defined medium. Cells were disrupted with glass-bead and RNA was isolated by a Trizol method. A custom Affymetrix chip was utilized for microarray analysis. Genes were considered differentially-expressed if they showed a >2.0-fold difference in expression between the strains and a Bayesian (Cyber-T) t-test value of <0.001. The posterior probability of differential expression (PPDE) (0.964.
Daniel Wilson Shelver
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