Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Oct 29 2008
Last update date: Mar 20 2012
Access: Public
Chemicals: Methionine
Taxon: Streptococcus agalactiae
Dataset link Streptococcus agalactiae genes controlled by the MtaR transcription factor

Experimental Protocol


Three cultures of an mtaR insertional mutant (DS101) and three cultures of the isogenic wild-type strain (COH1) were grown in a chemically-defined medium. Cells were disrupted with glass-bead and RNA was isolated by a Trizol method. A custom Affymetrix chip was utilized for microarray analysis. Genes were considered differentially-expressed if they showed a >2.0-fold difference in expression between the strains and a Bayesian (Cyber-T) t-test value of <0.001. The posterior probability of differential expression (PPDE) (0.964.

Repositories


GEO

GSE13325

ArrayExpress

E-GEOD-13325

BioProject

PRJNA109919

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Contact


Daniel Shelver
Daniel Wilson Shelver

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