|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Nov 27 2007|
|Last update date:||Jun 6 2014|
|Dataset link||Trophic Factor Gene Expression in Supporting Cells of the Crista Ampullaris:Implications for Hair Cell Regeneration|
Using laser capture microdisection, the more superficial layer of the cristae ampullaris sensory epithelium encompassing types I and II hair cells, and the supporting cell cytoplasm interspersed between them was captured in the first pass, and the underlying supporting cell bodies resting on the basal lamina were captured in a second pass. Individual hair cells were identified under light microscopy at 200X magnification by their stereociliary tuft and the calyceal nerve ending surrounding them and the presence of their nucleus in the outermost portion of the epithelia.