Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 23
Release date: Jun 15 2016
Last update date: Oct 18 2018
Access: Public
Dataset link Rocaglamide A converts RNA helicase eIF4A into a sequence-specific translational repressor

Experimental Protocol


Ribosome profiling, mRNA-Seq, RIP-Seq, and Bind-n-Seq Ribosome profiling for sample 1-5, and 11-15. Sample1 and 2 are replicates of control of DMSO treatment for sample 3-5, and 11, with RocA and PP242 treatments. Sample 12 and 13 are replicates of control of DMSO treatment for sample 14 and 15 with Hipp treatments. mRNA-Seq for sample 6-10. Sample 6 and 7 are replicates of control of DMSO treatment for sample 8-10 with RocA treatments. RIP-Seq for 16-19. Sample 16 and 17 are replicates of control of DMSO treatment for sample 18-19 with RocA treatments. Bind-n-Seq for 20-23. Sample 21 is control of DMSO treatment for sample 22-23 with RocA treatments. Sample 20 is a input contol for protein-bound fraction of sample 21. We stably expressed SBP (streptavidin binding peptide)-tagged eIF4A in HEK 293T-REx cells and purified it via M270 streptavidin beads (life techonologies).

Repositories


GEO

GSE70211

ArrayExpress

E-GEOD-70211

ENA

SRP059825

BioProject

PRJNA287879

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Contact


Nicholas Ingolia
Nicholas T Ingolia

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