Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Jul 1 2008
Last update date: Apr 28 2017
Access: Public
Diseases: Neoplasms, Neoplasms, Germ Cell and Embryonal
Chemicals: Ethanol, Estrogens, Tamoxifen
Genes: HDAC3, NCOR1, Ly2
Dataset link Deletion of Histone Deacetylase 3 reveals critical roles in S-phase progression and DNA damage control

Experimental Protocol


Initially, we compared RNA from Ad-Cre infected Hdac3FL/+ and Hdac3FL/- MEFs. In this analysis, RNA isolated from 64hr and 72hr post Ad-Cre infection were used. We extended the analysis to Hdac3FL/- MEFs expressing Cre-ER (tamoxifen responsive Cre-estrogen receptor fusion protein). In this analysis, two different MEF preparations (repl1 and repl2), two different time points (64hr and 72hr) were used for array analysis with tamoxifen-treated MEFs. For vehicle (ethanol) treated MEFs, two different MEF preparations (repl1 and repl2), and a single timepoint (64hr) was used.

Repositories


GEO

GSE10250

BioProject

PRJNA108437

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Contact


Srividya Bhaskara